Before embarking on her pop career, the chart-topping singer played Charlene Robinson in Neighbours for two years.
On Thursday morning, after it was announced that the Australian soap would be shutting down production for good later this year, Kylie tweeted about her fondness for the show that helped launch her to international stardom.
“We had no idea how big the show would become and how passionately viewers would take it to heart. Pure love!”
She added: “I can still hear Madge calling… CHARLENE!!!!”
Fellow Neighbours alum Guy Pearce also shared his sadness that the show would be coming to an end.
He tweeted: “I’m so sorry to hear Neighbours will end. Aside from being the incredible launchpad it’s been no one can deny the effect it’s had throughout millions of homes around the world. It’s bonded all of us in immeasurable ways.
“A true honour to have been a part of it. Great memories.”
Over the years, Neighbours also helped launch the careers of many other Australian stars, including Jason Donovan, Natalie Imbruglia, Liam Hemsworth and two-time Oscar nominee Margot Robbie.
It was first confirmed last month that the future of the long-running show was in jeopardy after UK broadcaster Channel 5 said they would stop airing Neighbours from this summer onwards.
Producers had been trying to find another broadcaster to help save the show, but confirmed on Thursday morning that this search was unsuccessful.
“We are so sorry to say that after nearly 37 years and almost 9000 episodes broadcast we have to confirm that Neighbours will cease production in June,” they said in a series of tweets.
“Following the loss of our key broadcast partner in the UK and despite an extensive search for alternative funding, we simply have no option but to rest the show.
“To our amazing, loyal fans, we know this is a huge disappointment, as it is to all of us on the team. We thank you for all your messages and support and promise to end the show on an incredible high.”