While Spurs have not named any of the impacted individuals, they are understood to include some of Conte’s most important and in-form players, with the head coach due to provide an update at a press conference at 2pm GMT on Wednesday.
Giovani Lo Celso is expected to be available again after a knee injury but may not be deemed ready to start.
If Kane is rested, the Dutch international could play through the middle, paving the way for Lo Celso to come in as one of the No10s.
Davinson Sanchez struggled as the left-sided centre-half against Mura but Conte may have little option but to play the Colombian there again.
A number of young players, including Dilan Markanday, are training with the first team and Conte is likely to have to name a youthful bench.
Rennes have already topped the Europa Conference League group and could rest players, while Spurs need a result to keep their hopes of finishing as runners-up – and going into a play-off next year – alive.
Tottenham predicted XI (3-4-2-1): Gollini; Tanganga, Rodon, Sanchez; Doherty, Winks, Hojbjerg, Reguilon; Bergwijn, Dele; Kane.