The Dutch finished top of Group C, winning all three of their games, and with a relatively favourable draw, look to have a strong chance of going deep at their first major tournament since the 2014 World Cup.
The Czechs, meanwhile, got their tournament off to a flying start by beating Scotland at Hampden Park, but a draw with Croatia and defeat to England at Wembley meant they had to settle for a best third-place finisher slot into the last 16.
Where to watch Netherlands vs Czech Republic
TV channel: Today’s match will be televised live on ITV for free, with kick-off at 5pm BST.
Live stream: UK viewers will also be able to watch the game online for free via the ITV Hub.
Live coverage: Standard Sport will bring you all the match action, analysis and reaction.
Read our dedicated match preview here.