England boss Gareth Southgate has printed out the national anthem and called on his Three Lions stars to “sing with pride”.
Wembley will have 45,000 fans present for the last 16 clash against Germany on Tuesday night.
Southgate believes a powerful rendition of the anthem could give them the edge early on.
Before England’s Euro 96 semi-final against Germany at the old Wembley players and fans alike belted out ‘God Save the Queen’ in what has become an iconic moment.
Before last week’s 1-0 win over the Czech Republic, TV cameras showed every member of England’s starting XI singing the anthem.
Dominic Calvert-Lewin started on the bench for the Group D victory but has revealed he got goose bumps when he sung it with fans present.
He said: “It’s an unbelievable. My first experience playing for England with fans was the friendly against Austria this month.
“That was the first time I heard the National Anthem as a player and got to sing it as a player with fans in the stadium — it gave me goose bumps.
“So playing in the Euros, with 40-odd thousand singing it in the stadium, it’s going to make a massive difference.
“It gives you that extra boost and it’s just a very proud moment.”
Southgate is certainly a patriotic individual and has looked to unite the team and its fans ever since he took the England job.
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The 50-year-old hammered home to his players that they would be under the spotlight when it came to singing the national anthem and it was up to them to underline their pride at playing for England.
A source told the Sun : “In previous tournaments, players have been seen half-heartedly mumbling the lyrics — some even remaining closed-mouthed.
“Gareth is hugely patriotic and feels it’s essential that players foster a sense of national pride by learning and singing the words.
“He sat the players down before the tournament and explained cameras would be on them the whole time and that it was essential to show fans just what it means to pull on an England shirt.
“If the players sing, it inspires the crowd and those watching at home.”
The source added: “There has been some ribbing for the more tone-deaf players but it’s been great for team spirit.”
England were knocked out at major tournaments by Germany in 2010 and 1996.
They go into the game as narrow favourites however and would face either Ukraine or Sweden were they to make the last eight.