The Wales prop is struggling with a shoulder issue and he will be replaced in the starting XV by Rory Sutherland.
Sutherland’s promotion into the starting team means that Vunipola has been drafted into the match-day squad.
The Lions say Jones is struggling with a “minor shoulder injury” picked up in training on Thursday this week.
“It’s really bad luck for Wyn and we all feel for him missing out on tonight’s game,” said Warren Gatland.
“However, we’re confident he’ll be back in training next week and have no plans to bring in anyone else at this stage.”
England and Saracens prop Vunipola brings plenty of experience to the party, having toured with the Lions back in 2013 and 2017.